Composite
Part:BBa_K4998046:Design
Designed by: Rafail Andreou Group: iGEM23_thessaloniki (2023-10-10)
hetR composite
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The need for a constant, strong promoter that is compatible with the cyanobacterial strains led to the choice of psbA1.
Source
psbA1 promoter: BBa_K4998031, RBS: BBa_K103015, hetR gene: BBa_K4998032, trp terminator: BBa_K4998032